{"id":1779,"date":"2021-10-10T13:58:29","date_gmt":"2021-10-10T13:58:29","guid":{"rendered":"https:\/\/science.sjp.ac.lk\/zoo\/?p=1779"},"modified":"2021-10-15T14:04:19","modified_gmt":"2021-10-15T14:04:19","slug":"isolation-and-identification-of-cellulase-producing-and-sugar-fermenting-bacteria-for-second-generation-bioethanol-production","status":"publish","type":"post","link":"https:\/\/science.sjp.ac.lk\/zoo\/2021\/10\/10\/isolation-and-identification-of-cellulase-producing-and-sugar-fermenting-bacteria-for-second-generation-bioethanol-production\/","title":{"rendered":"Isolation and Identification of Cellulase Producing and Sugar Fermenting Bacteria for Second-Generation Bioethanol Production"},"content":{"rendered":"<p>&nbsp;<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"alignnone size-full wp-image-1780\" src=\"https:\/\/science.sjp.ac.lk\/zoo\/wp-content\/uploads\/sites\/20\/2021\/10\/biofuel.jpg\" alt=\"biofuel\" width=\"1000\" height=\"625\" srcset=\"https:\/\/science.sjp.ac.lk\/zoo\/wp-content\/uploads\/sites\/20\/2021\/10\/biofuel.jpg 1000w, https:\/\/science.sjp.ac.lk\/zoo\/wp-content\/uploads\/sites\/20\/2021\/10\/biofuel-40x25.jpg 40w, https:\/\/science.sjp.ac.lk\/zoo\/wp-content\/uploads\/sites\/20\/2021\/10\/biofuel-300x188.jpg 300w, https:\/\/science.sjp.ac.lk\/zoo\/wp-content\/uploads\/sites\/20\/2021\/10\/biofuel-768x480.jpg 768w, https:\/\/science.sjp.ac.lk\/zoo\/wp-content\/uploads\/sites\/20\/2021\/10\/biofuel-160x100.jpg 160w\" sizes=\"(max-width: 1000px) 100vw, 1000px\" \/><\/p>\n<p>Authors: L.I. Weerasinghe, Tharindu Madusanka\u00a0and Pathmalal M. Manage<\/p>\n<p>Journal: <em>International Journal of Renewable Energy Development<\/em><\/p>\n<p>Over the last decades, the negative impacts of fossil fuel on the environment and increasing demand for energy due to the unavoidable depletion of fossil fuels, has transformed the world\u2019s interests towards alternative fuels. In particular, bioethanol production from cellulosic biomass for the transportation sector has been incrementing since the last decade. The bacterial pathway for bioethanol production is a relatively novel concept and the present study focused on the isolation of potential \u201ccellulase-producing\u201d bacteria from cow dung, compost soil, and termite gut and isolating sugar fermenting bacteria from palm wine. To select potential candidates for cellulase enzyme production, primary and secondary assays were conducted using the Gram\u2019s iodine stain in Carboxy Methyl Cellulose (CMC) medium and the Dinitrosalicylic acid (DNS) assays, respectively. Durham tube assay and Solid-Phase Micro-Extraction (SPME) coupled with Gas Chromatography-Mass Spectrometry (GC-MS) was used to evaluate the sugar fermenting efficiency of the isolated bacteria. Out of 48 bacterial isolates, 27 showed cellulase activity where\u00a0<em>Nocardiopsis<\/em>\u00a0sp. (S-6) demonstrated the highest extracellular crude enzyme activity of endoglucanase (1.56\u00b10.021 U) and total cellulase activity (0.93\u00b10.012 U). The second-highest extracellular crude enzyme activity of endoglucanase (0.21\u00b10.021 U) and total cellulase activity (0.35\u00b10.021 U) was recorded by\u00a0<em>Bacillus<\/em>\u00a0sp. (T-4). Out of a total of 8 bacterial isolates,\u00a0<em>Achromobacter\u00a0<\/em>sp. (PW-7) was positive for sugar fermentation resulting in 3.07% of ethanol in broth medium at 48 h incubation. The results of the study revealed that\u00a0<em>Nocardiopsis<\/em>\u00a0sp. (S-6) had the highest cellulase enzyme activity. However, the highest ethanol percentage was achieved with by having both\u00a0<em>Bacillus\u00a0<\/em>sp<em>.<\/em>\u00a0(T-4) and\u00a0<em>Achromobacter sp.<\/em>\u00a0(PW-7) for the simultaneous saccharification and fermentation (SSF) method, as compared to separate hydrolysis and fermentation (SHF) methodologies<\/p>\n","protected":false},"excerpt":{"rendered":"<p>&nbsp; Authors: L.I. Weerasinghe, Tharindu Madusanka\u00a0and Pathmalal M. Manage Journal: International Journal of Renewable Energy Development Over the last decades, the negative impacts of fossil fuel on the environment and &hellip; <a href=\"https:\/\/science.sjp.ac.lk\/zoo\/2021\/10\/10\/isolation-and-identification-of-cellulase-producing-and-sugar-fermenting-bacteria-for-second-generation-bioethanol-production\/\" class=\"more-link\">Continue reading <span class=\"screen-reader-text\">Isolation and Identification of Cellulase Producing and Sugar Fermenting Bacteria for Second-Generation Bioethanol Production<\/span><\/a><\/p>\n","protected":false},"author":32,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_ti_tpc_template_sync":false,"_ti_tpc_template_id":"","footnotes":""},"categories":[7],"tags":[],"_links":{"self":[{"href":"https:\/\/science.sjp.ac.lk\/zoo\/wp-json\/wp\/v2\/posts\/1779"}],"collection":[{"href":"https:\/\/science.sjp.ac.lk\/zoo\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/science.sjp.ac.lk\/zoo\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/science.sjp.ac.lk\/zoo\/wp-json\/wp\/v2\/users\/32"}],"replies":[{"embeddable":true,"href":"https:\/\/science.sjp.ac.lk\/zoo\/wp-json\/wp\/v2\/comments?post=1779"}],"version-history":[{"count":1,"href":"https:\/\/science.sjp.ac.lk\/zoo\/wp-json\/wp\/v2\/posts\/1779\/revisions"}],"predecessor-version":[{"id":1781,"href":"https:\/\/science.sjp.ac.lk\/zoo\/wp-json\/wp\/v2\/posts\/1779\/revisions\/1781"}],"wp:attachment":[{"href":"https:\/\/science.sjp.ac.lk\/zoo\/wp-json\/wp\/v2\/media?parent=1779"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/science.sjp.ac.lk\/zoo\/wp-json\/wp\/v2\/categories?post=1779"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/science.sjp.ac.lk\/zoo\/wp-json\/wp\/v2\/tags?post=1779"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}