{"id":1798,"date":"2021-03-08T11:50:10","date_gmt":"2021-03-08T11:50:10","guid":{"rendered":"https:\/\/science.sjp.ac.lk\/zoo\/?p=1798"},"modified":"2021-10-23T11:53:24","modified_gmt":"2021-10-23T11:53:24","slug":"compensatory-base-changes-reveal-sexual-incompatibility-among-members-of-the-anopheles-subpictus-sensu-lato-diptera-culicidae-species-complex-in-sri-lanka","status":"publish","type":"post","link":"https:\/\/science.sjp.ac.lk\/zoo\/2021\/03\/08\/compensatory-base-changes-reveal-sexual-incompatibility-among-members-of-the-anopheles-subpictus-sensu-lato-diptera-culicidae-species-complex-in-sri-lanka\/","title":{"rendered":"Compensatory Base Changes Reveal Sexual Incompatibility among Members of the Anopheles subpictus Sensu Lato (Diptera: Culicidae) Species Complex in Sri Lanka"},"content":{"rendered":"<p><img decoding=\"async\" loading=\"lazy\" class=\"alignnone size-full wp-image-1799\" src=\"https:\/\/science.sjp.ac.lk\/zoo\/wp-content\/uploads\/sites\/20\/2021\/10\/life-11-00211-g004-550.jpg\" alt=\"life-11-00211-g004-550\" width=\"550\" height=\"267\" srcset=\"https:\/\/science.sjp.ac.lk\/zoo\/wp-content\/uploads\/sites\/20\/2021\/10\/life-11-00211-g004-550.jpg 550w, https:\/\/science.sjp.ac.lk\/zoo\/wp-content\/uploads\/sites\/20\/2021\/10\/life-11-00211-g004-550-40x19.jpg 40w, https:\/\/science.sjp.ac.lk\/zoo\/wp-content\/uploads\/sites\/20\/2021\/10\/life-11-00211-g004-550-300x146.jpg 300w\" sizes=\"(max-width: 550px) 100vw, 550px\" \/><\/p>\n<p>Authors:\u00a0D. P. W. Jayatunga,I. N. Harischandra,N. V. Chandrasekhara and B. G. D. N. K. de Silva<\/p>\n<p>Journal:\u00a0Life<\/p>\n<p>The mosquito\u00a0<span class=\"html-italic\">Anopheles (Cellia) subpictus<\/span>\u00a0sensu lato (s.l.) is a major secondary vector of malaria in Sri Lanka. The sibling species composition in this species complex in Sri Lanka remains debatable. Compensatory base changes (CBCs) in the secondary structures of internal transcribed spacer 2 (ITS2) are reliable sources to predict sexual incompatibility among closely related species. The objective of the present study was to investigate the\u00a0<span class=\"html-italic\">An. subpictus<\/span>\u00a0s.l. populations in Sri Lanka using the CBC analysis. Mosquito DNA was amplified and sequenced for the ITS2 region. The sequences were annotated using ITS2 Database. ITS2 secondary structures were constructed and analyzed for CBCs using various bioinformatics tools. The ITS2 regions consisted of two different lengths, 575 bp and 480 bp. The two CBCs and three hemi CBCs identified in the present study suggest that there may be at least two sexually incompatible sibling species. In conclusion, it is likely that there may be only two reproductively isolated sibling species in the\u00a0<span class=\"html-italic\">An. subpictus<\/span>\u00a0species complex in Sri Lanka. However, due to high divergence of ITS2 in these species, it is reasonable to assume that they may be undergoing a speciation event to separate as a distinct species.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Authors:\u00a0D. P. W. Jayatunga,I. N. Harischandra,N. V. Chandrasekhara and B. G. D. N. K. de Silva Journal:\u00a0Life The mosquito\u00a0Anopheles (Cellia) subpictus\u00a0sensu lato (s.l.) is a major secondary vector of malaria &hellip; <a href=\"https:\/\/science.sjp.ac.lk\/zoo\/2021\/03\/08\/compensatory-base-changes-reveal-sexual-incompatibility-among-members-of-the-anopheles-subpictus-sensu-lato-diptera-culicidae-species-complex-in-sri-lanka\/\" class=\"more-link\">Continue reading <span class=\"screen-reader-text\">Compensatory Base Changes Reveal Sexual Incompatibility among Members of the Anopheles subpictus Sensu Lato (Diptera: Culicidae) Species Complex in Sri Lanka<\/span><\/a><\/p>\n","protected":false},"author":32,"featured_media":1799,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_ti_tpc_template_sync":false,"_ti_tpc_template_id":"","footnotes":""},"categories":[7],"tags":[],"_links":{"self":[{"href":"https:\/\/science.sjp.ac.lk\/zoo\/wp-json\/wp\/v2\/posts\/1798"}],"collection":[{"href":"https:\/\/science.sjp.ac.lk\/zoo\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/science.sjp.ac.lk\/zoo\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/science.sjp.ac.lk\/zoo\/wp-json\/wp\/v2\/users\/32"}],"replies":[{"embeddable":true,"href":"https:\/\/science.sjp.ac.lk\/zoo\/wp-json\/wp\/v2\/comments?post=1798"}],"version-history":[{"count":1,"href":"https:\/\/science.sjp.ac.lk\/zoo\/wp-json\/wp\/v2\/posts\/1798\/revisions"}],"predecessor-version":[{"id":1800,"href":"https:\/\/science.sjp.ac.lk\/zoo\/wp-json\/wp\/v2\/posts\/1798\/revisions\/1800"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/science.sjp.ac.lk\/zoo\/wp-json\/wp\/v2\/media\/1799"}],"wp:attachment":[{"href":"https:\/\/science.sjp.ac.lk\/zoo\/wp-json\/wp\/v2\/media?parent=1798"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/science.sjp.ac.lk\/zoo\/wp-json\/wp\/v2\/categories?post=1798"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/science.sjp.ac.lk\/zoo\/wp-json\/wp\/v2\/tags?post=1798"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}