solation and Molecular Characterization of Polycyclic Aromatic Hydrocarbon Degrading Bacteria from Effluent Water from Weras River Park, Sri Lanka

Authors: A.A. Prathiba, F. Sumaiya Idroos and Pathmalal M. Manage

Journal: Environment and Natural Resources Journal

The present study records the detection of PAHs such as naphthalene and anthracene and isolation of PAHs degrading bacteria from a restaurant site, Weras River Park, in Boralesgamuwa, Sri Lanka. Water samples were collected in seven locations of the study area. Water temperature (oC), pH, and electric conductivity (EC) were measured at the site itself using standard meters. Nitrogen nitrate (N-NO3-) and total phosphate (TP) were measured at the laboratory following the standard methods. Following the extraction of PAHs in collected water samples, detection was carried out using the PDA-HPLC diode array method. PAH degrading bacteria were identified using microplate assay. The selected bacteria strains were subjected to degradation kinetic studies following molecular identification by 16S rRNA analysis. Phylogenetic analysis identified Achromobacter spanius as potential naphthalene degrading bacteria, where Alcaligens faecalis was recorded as an anthracene degrader. Degradation study confirmed that A. spanius efficiently degraded naphthalene at the rate of 0.145±0.002 ppm/day, whereas A. faecalis degraded anthracene at the rate of 0.181±0.036 ppm/day, respectively. Degradation of structures of the Naphthalene and Anthracene by A. spanius and A. faecalis was further analyzed by Fourier Transform Infrared Spectroscopy (FTIR). This is the first record on naphthalene degradation by the bacterium A. spanius.

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Cnidarian toxins: recent evidences for potential therapeutic uses

Authors: J.M.N.J.Jayathilake, K.V.K Gunathilake

Journal: The European Zoological Journal

Marine toxins have received global attention for their involvement in human intoxication. Many marine phyla are well adapted to produce venoms or toxins protect themselves from associated micro fauna, predators and pathogens. Despite the toxicity, some bio toxins stand as potential drug leads in human and veterinary medicine. Amongst all marine fauna, Cnidarians are well renowned for producing bioactive peptides which are used in drug development, as they harbor various biological activities; anticancer, anti-inflammatory, immunomodulatory, radical scavenging, anti-parasitic activities, etc. Particularly, this review summarizes the bioactivities recorded from Cnidarian toxins and the possibility of using them as therapeutic agents, leading to develop into commercial products in the future.

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Mitochondrial metabolic genes provide phylogeographic relationships of global collections of Aedes aegypti (Diptera: Culicidae)

Authors: H. S. D. Fernando, Menaka Hapugoda, Rushika Perera, William C. Black, B. G. D. N. K. De Silva

Journal: PLoS ONE

Phylogeographic relationships among global collections of the mosquito Aedes aegypti were evaluated using the mitochondrial Cytochrome C Oxidase 1 (CO1) and NADH dehydrogenase subunit 4 (ND4) genes including new sequences from Sri Lanka. Phylogeographic analysis estimated that Ae. aegypti arose as a species ~614 thousand years ago (kya) in the late Pleistocene. At 545 kya an “early” East African clade arose that continued to differentiate in East Africa, and eventually gave rise to three lineages one of which is distributed
throughout all tropical and subtropical regions, a second that contains Southeast Asian/Sri Lankan mosquitoes and a third that contains mostly New World mosquitoes. West African collections were not represented in this early clade. The late clade continued to differentiate
throughout Africa and gave rise to a lineage that spread globally. The most recent branches of the late clade are represented by South-East Asia and India/Pakistan collections. Analysis of migration rates suggests abundant gene flow between India/Pakistan and the rest of the world with the exception of Africa.

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Bows and arrows and complex symbolic displays 48,000 years ago in the South Asian tropics

Authors: Michelle C. Langley, Noel Amano, Oshan Wedage, Siran Deraniyagala, M.M Pathmalal, Nimal Perera, Nicole Boivin, Michael D. Petraglia, Patrick Roberts
Journal: SCIENCE ADVANCES

Archaeologists contend that it was our aptitude for symbolic, technological, and social behaviors that was central to Homo sapiens rapidly expanding across the majority of Earth’s continents during the Late Pleistocene. This expansion included movement into extreme environments and appears to have resulted in the displacement of numerous archaic human populations across the Old World. Tropical rainforests are thought to have been particularly challenging and, until recently, impenetrable by early H. sapiens. Here, we describe evidence for bow-and-arrow hunting toolkits alongside a complex symbolic repertoire from 48,000 years before present at the Sri Lankan site of Fa-Hien Lena—the earliest bow-and-arrow technology outside of Africa. As one of the oldest H. sapiens rainforest sites outside of Africa, this exceptional assemblage provides the first detailed insights into how our species met the extreme adaptive challenges that were encountered in Asia during global expansion

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